BROWN FK-棕色FK
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ID： |
D43C03675A794D7187EA858E5959C64C |
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文件大小(MB)： |
0.12 |
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页数： |
6 |
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文件格式： |
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日期： |
2008-7-13 |
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购买： |
文本摘录（文本识别可能有误，但文件阅览显示及打印正常，pdf文件可进行文字搜索定位）：
BROWN FK,Prepared at the 30th JECFA (1986), published in FNP 37 (1986) and in FNP 52 (1992). Metals and arsenic specifications revised at the 59th JECFA (2002). No ADI was allocated at the 30th (JECFA 1986),SYNONYMS,CI Food Brown 1; INS No. 154,DEFINITION,A mixture of six mono-, bis- and trisazo colours (see ‘Chemical names’ below) and subsidiary colouring matters together with water, sodium chloride and/or sodium sulfate as the principal uncoloured components.,This product as manufactured, and to which these specifications apply, is often diluted with sodium chloride to a total colouring matters content of about 50% to meet the needs of users.,Chemical names,A mixture of,I Sodium 4-(2,4-diaminophenylazo) benzenesulfonate,II Sodium 4-(4,6-diamino-m-tolylazo) benzenesulfonate,III Disodium 4,4'-(4,6-diamino-1,3-phenylenebisazo)- di(benzenesulfonate),IV Disodium 4,4'-(2,4-diamino-1,3-phenylenebisazo)- di(benzenesulfonate),V Disodium 4,4'-(2,4-diamino-5-methyl-1,3-phenylene- bisazo)di(benzene-sulfonate),VI Trisodium 4,4',4''-(2,4-diaminobenzene-1,3,5- trisazo)tri-(benzene-sulfonate),C.A.S. number,8062-14-4,Chemical formula,I C12H11N4NaO3S,II C13H13N4NaO3S,III C18H14N6Na2O6S2,IV C18H14N6Na2O6S2,V C19H16N6Na2O6S2,VI C24H17N8Na3O9S3,Structural formula,I,II,III,IV,V,VI,Formula weight,I 314.30,II 328.33,III 520.46,IV 520.46,V 534.47,VI 726.59,Assay,Not less than 70% total colouring matters. Of the total colouring matters present the proportions of the components shall not exceed:,I 26%,II 17%,III 17%,IV 16%,V 20%,VI 16%,DESCRIPTION,Red-brown powder or granules,FUNCTIONAL USES,Colour,CHARACTERISTICS,IDENTIFICATION,Solubility (Vol. 4),Soluble in water; sparingly soluble in ethanol,Identification of colouring matters (Vol. 4),Passes test,PURITY,Loss on drying at 135o,(Vol. 4),Not more than 30% together with chloride and sulfate calculated as sodium salts,Water insoluble matter,(Vol. 4),Not more than 0.2%,Lead (Vol. 4),Not more than 2 mg/kg,Determine using an atomic absorption technique appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the method described in Volume 4, “Instrumental Methods.”,Subsidiary colouring matters,Not more than 3.5%,See description under METHOD OF ASSAY,Organic compounds other than colouring matters,Not more than,- 0.7% of 4-aminobenzene-1-sulfonic acid,- 0.35% of - m-phenylenediamine and 4-methyl-m-phenylenediamine,See description under TESTS,Unsulfonated primary aromatic amines (Vol. 4),Not more than 0.007% calculated as aniline (other than m-phenylene diamine and 4-methyl-m-phenylene diamine),Ether extractable matter,(Vol. 4),Not more than 0.2%,TESTS,Organic compounds other,Use liquid chromatography (see Volume 4) under the following conditions:,than colouring matters,Detector: A UV HPLC detector recording absorbances at 254 nm,Column: 250 x 4 mm Li Chromosorb RP8, 7 μm,Solvent system,A: 0.075 M sodium acetate solution adjusted to pH 6.0 using glacial acetic acid.,B: A:methanol (2:3),Flow rate: 1 ml/min,Gradient:,0 min: 100% (A), 0% (B),15 min: 0% (A), 100% (B),20 min: 0% (A), 100% (B),25 min: 100% (A), 0% (B),METHOD OF,ASSAY,Determination of the amount of component colouring matters and subsidiary colouring matters.,Apparatus,- Glass tank capable of holding 20 cm x 20 cm glass TLC plates.,- Micrometer syringe capable of delivering 0.10 ml with a tolerance of ± 0.002 ml,- Spectrophotometer,Reagents:,All of recognized analytical grade,- Chromatography solvent: A mixture of 4 parts phenol and 1 part water by volume,- Diethyl ether,- Kieselgel G,- Extraction solvent: Mix 100 ml 10% sodium carbonate solution with 500 ml methanol and dilute to 1 L with water,Procedure,Prepare a 20 x 20 cm TLC plate with a 0.5 mm thick coating of Kieselgel G. Using the micrometer syringe apply a solution containing 0.4 mg Brown FK as evenly as possible in an area near the bottom of the plate. Develop the chromatogram in the phenol/water mixture allowing the solvent to ascend the full height of the plate; then remove it from the tank and allow it to dry.,The diagram shows a typical Brown FK chromatogram with the bands numerically identified.,Remove each band from the plate and transfer it to a small beaker. The subsidiary colouring matters are located in the area between bands I and V. Remove this area of Kieselgel G. Transfer it to a beaker and wash it with a,small quantity of ether to remove the phenol. Allow the residual ether to evaporate.,Add 10 ml of extraction solvent to each beaker and swirl to extract the colour. Filter through a small filter paper and measure the absorbance versus extraction s……
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